Gstm1 Knockout cell line (RAW 264.7)

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Gstm1 Knockout cell line (RAW 264.7) Order

Gstm1 Knockout cell line (RAW 264.7)
Catalog#
YKO-M276
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Gstm1 Knockout cell line (RAW 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Gstm1 Knockout cell line (RAW 264.7)
Cell Morphology
monocyte/macrophage,adherent
Passage ratio
1:2-1:3
Species
Gene Name
Gstm1
Gene ID
14862
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 4 (based on transcript Gstm1-202)
Validation: PCR+Sanger Sequencing
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:RAW 264.7		 STR Info (Cell bank)
Cell Line:RAW 264.7
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 18.018.0
4-2 22.322.3
6-7 12.012.0
19-2
1-2 17.0
7-1 25.225.2
8-1 13.013.0
1-1 15.016.0
3-2 14.014.0
2-1 16.016.0
15-3 22.322.3
6-4 18.018.0
13-1 16.216.2
11-2 17.017.0
17-2 14.016.014.016.0
12-1 16.016.0
5-5 14.014.0
X-1 24.024.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Gstm1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RAW 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Gstm1 gene knockout Gstm1 Knockout cell line (RAW 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Gstm1 Knockout cell line (RAW 264.7)
Gstm1 Knockout cell line (RAW 264.7)
Catalog#
YKO-M276
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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